Dionex Ultimate 3000 HPLC System (Refurbished)

The Dionex UltiMate 3000 HPLC System by Thermo Fisher Scientific is a modular high-performance liquid chromatography (HPLC) platform used in analytical, pharmaceutical, and research laboratories for the separation, identification, and quantification of complex chemical compounds. 

This complete system includes an HPLC pump, autosampler, flow manager, and solvent rack, providing a ready-to-integrate chromatography setup for routine and advanced laboratory applications.

The UltiMate 3000 series is a modular HPLC and UHPLC platform designed by Thermo Scientific for high-resolution liquid chromatography. 

Instead of a single fixed unit, the system is built from independent, interoperable modules: a pump, autosampler, flow manager, detector, and solvent management components that work together under Chromeleon Chromatography Data System (CDS) software. 

This modular architecture allows laboratories to configure the system around specific analytical needs, from routine quality control testing to complex research-grade separations.

This listing includes the core modules needed to run a functional HPLC workflow:

• 1x Dionex UltiMate 3000 HPLC System (base unit)
• 1x HPLC Flow Manager: FLM-3100B
• 1x HPLC Autosampler: WPS-3000TB (Well Plate Sampler)
• 1x Solvent Rack / Degasser: SRD-3600
• 1x HPLC Pump: DGP-3600MB (Dual Gradient Micro Pump)

Technical Specifications

Part numbers and serial numbers above are taken directly from the manufacturer nameplates on the physical unit.

Key Benefits

• Modular flexibility components can be swapped, upgraded, or reconfigured as lab requirements evolve
• High chromatographic precision delivers consistent, reproducible peak separation for reliable analytical results
• Efficient solvent handling the integrated solvent rack supports stable, low-noise mobile phase delivery
• Automated sample handling the autosampler reduces manual intervention and improves throughput
• Proven industry platform widely used across pharmaceutical, environmental, food safety, and academic research labs
• Scalable performance suitable for both routine HPLC workflows and more demanding separation applications

System Components & Features

HPLC Pump: DGP-3600MB

A dual gradient micro pump that delivers precise, pulse-free solvent flow across a range of flow rates, forming the core of accurate and reproducible gradient or isocratic separations.

HPLC Autosampler: WPS-3000TB

A well plate sampler that automates sample injection with high precision, minimising manual handling errors and enabling unattended, sequential samples runs across well-plate formats.

Flow Manager: FLM-3100B

Regulates and coordinates solvent flow and switching valves between system modules, supporting stable temperature control and consistent delivery throughout each analytical run.

Solvent Rack / Degasser: SRD-3600

Provides organised, integrated storage and inline degassing of mobile phase solvents, removing dissolved gases to support stable, low-noise gradient delivery.

Use Cases

• Pharmaceutical QA/QC verifying drug purity, potency, and impurity profiles
• Environmental testing detecting contaminants and pollutants in water, soil, and air samples
• Food & beverage analysis testing for additives, contaminants, and nutritional compounds
• Academic and research labs method development and complex compound separation
• Biotechnology protein and biomolecule analysis workflows

Why Choose This Dionex UltiMate 3000 System

This configuration gives buyers a complete, functional HPLC workflow in one package pump, autosampler, flow manager, and solvent rack rather than requiring separate sourcing of individual modules. 

It’s a practical option for labs looking to get an UltiMate 3000 system running quickly without piecing together supported components from multiple sellers.

Frequently Asked Questions

What is the Dionex UltiMate 3000 HPLC System used for? 

It’s used for high-performance liquid chromatography separating, identifying, and quantifying compounds in samples across pharmaceutical, environmental, food safety, and research applications.

What modules are included with this Dionex UltiMate 3000 system? 

This system includes four core modules: the DGP-3600MB dual gradient micro pump, the WPS-3000TB well plate autosampler, the FLM-3100B flow manager, and the SRD-3600 solvent rack/degasser.

What is the part number of the Dionex UltiMate 3000 pump in this system? 

The pump module is a DGP-3600MB (Dual Gradient Micro Pump), part number 5037.0060.

What is the part number of the autosampler? 

The autosampler is a WPS-3000TB Well Plate Sampler, part number 5821.0020.

Is the Dionex UltiMate 3000 a single unit or a modular system? 

It’s a modular platform. Each function pumping, sampling, detection, and solvent handling is a separate module that connects to form a complete HPLC system, which allows for flexible configuration and future upgrades.

What software runs the Dionex UltiMate 3000 HPLC System? 

The system is controlled through Thermo Scientific’s Chromeleon Chromatography Data System (CDS) software.

Can this system be upgraded or reconfigured later? 

Yes. Because of its modular design, individual components (such as the detector or pump) can be added, replaced, or upgraded independently without replacing the entire system.

Who manufactures the Dionex UltiMate 3000? 

It is manufactured by Thermo Fisher Scientific under the Dionex product line.